Table 2.
Specific repression of CPBF6 by gene silencinga
| Gene | CPBF6gs strain | Control strain | Fold change |
|---|---|---|---|
| CPBF1 | 3,110 (±1,010) | 2,780 (±268) | 1.12 |
| CPBF2 | 135 (±15.4) | 141 (±16.7) | 0.95 |
| CPBF3 | 638 (±45.0) | 482 (±18.4) | 1.32 |
| CPBF4 | 1,120 (±347) | 1,040 (±269) | 1.08 |
| CPBF5 | 127 (±44.0) | 136 (±38.9) | 0.93 |
| CPBF6 | 1.58 (±0.75) | 3,820 (±87.8) | 4.13 × 10−4 |
| CPBF7 | 1,730 (±282) | 1,540 (±190) | 1.13 |
| CPBF8 | 5,620 (±556) | 5,630 (±454) | 1.00 |
| CPBF9 | 311 (±24.9) | 248 (±30.7) | 1.26 |
| CPBF10 | 123 (±90.2) | 94.5 (±26.8) | 1.30 |
| CPBF11 | 96.3 (±14.6) | 77.0 (±10.7) | 1.25 |
a
Total RNA was extracted from the CPBF6gs and control strains, and expression analysis was performed using a custom-made E. histolytica DNA microarray. Average (± standard deviation) normalized signal intensities from DNA microarrays were determined in triplicate and indicate relative levels of the transcripts, in arbitrary units, of CPBF genes (CPBF1 to CPBF11) in the CPBF6gs and control strains. The fold change indicates the relative change in signal intensity in the CPBF6gs strain relative to that in the control strain.