Fig 6.

Transfection induced expression of parasite proteins on the surface of COS-7 cells. Expression of PvDBPII and PvMSP1P C-terminal fragments on the surface of COS-7 cells transfected with pEGFP-HSVgD1_PvDBPII and PvMSP1P-30, -42, -33, and -19 plasmid DNA was detected by IFA. Green fluorescent protein (GFP) (green, control), Alexa Fluor 543-conjugated goat anti-mouse antibody (red, MSP1P fragments), or Alexa Fluor 568-conjugated goat anti-rabbit antibody (red, DBPII) was used and visualized by confocal microscopy. Mouse antiserum against PBS was used as a negative control.