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Live L3 do not alter the function of TLR3 or TLR4 in LC. LC were cultured in medium alone or with live L3 for 24 h. The cells were harvested, and viable cells were further activated with medium alone, the TLR4 ligand LPS (A), or the TLR3 ligand poly(I · C) (B) for an additional 48 h. Production of IL-1β, IFN-γ, and IL-12p70 was measured in the culture supernatant with Luminex, and IL-6 and IL-10 levels were measured by ELISA. Each line represents an independent donor (n = 6 to 9).
