Table 1.
Soluble factors from L3 minimally alter gene expression in epidermal LCa
| Probe identifier (ID) | Gene name | Entrez gene name | Fold change (median) |
|---|---|---|---|
| ILMN_1702696 | AKR7L | Aldo-keto reductase family 7-like | 3.35 |
| ILMN_1706418 | EHMT1 | Euchromatic histone-lysine N-methyltransferase 1 | 3.78 |
| ILMN_1728353 | EYA3 | Eyes absent homolog 3 (Drosophila) | 2.52 |
| ILMN_1764605 | FGF6 | Fibroblast growth factor 6 | 2.93 |
| ILMN_1673380 | GNG12 | Guanine nucleotide binding protein (G protein), gamma 12 | 2.84 |
| ILMN_1723486 | HK2 | Hexokinase 2 | 2.98 |
| ILMN_1806165 | HSPA6 | Heat shock 70-kDa protein 6 (HSP70B′) | 4.09 |
| ILMN_1801632 | KRT5 | Keratin 5 | 2.82 |
| ILMN_1807300 | PKD2 (includes EG:18764) | Polycystic kidney disease 2 (autosomal dominant) | 2.17 |
| ILMN_2131103 | PPP1R3B | Protein phosphatase 1, regulatory subunit 3B | 3.93 |
| ILMN_1837297 | Homo sapiens cDNA clone UI-E-EJ1-aje-c-15-0-UI 5 | 1.81 | |
| ILMN_1855493 | Homo sapiens cDNA clone UI-E-EJ1-ajv-h-04-0-UI 3 | 2.79 | |
| ILMN_1796808 | Deleted | 2.41 | |
| ILMN_1713732 | ABL1 | c-abl oncogene 1, nonreceptor tyrosine kinase | −2.45 |
| ILMN_1698369 | AGAP6 (includes others) | ArfGAP with GTPase domain, ankyrin repeat and PH domain 5 | −2.15 |
| ILMN_2395055 | ATPAF1 | ATP synthase mitochondrial F1 complex assembly factor 1 | −2.76 |
| ILMN_1786433 | BCCIP | BRCA2- and CDKN1A-interacting protein | −2.46 |
| ILMN_1771326 | C15orf44 | Chromosome 15 open reading frame 44 | −3.47 |
| ILMN_2078334 | CNOT10 | CCR4-NOT transcription complex, subunit 10 | −3.39 |
| ILMN_1713321 | CYP20A1 | Cytochrome P450, family 20, subfamily A, polypeptide 1 | −3.69 |
| ILMN_1763129 | DCTPP1 | dCTP pyrophosphatase 1 | −2.37 |
| ILMN_1735461 | DDX21 | DEAD (Asp-Glu-Ala-Asp) box helicase 21 | −2.85 |
| ILMN_2145280 | DEF6 | Differentially expressed in FDCP 6 homolog (mouse) | −2.38 |
| ILMN_2112049 | DNLZ | DNL-type zinc finger | −3.61 |
| ILMN_1680130 | DYM | Dymeclin | −4.40 |
| ILMN_1751492 | FAM18B1 | Family with sequence similarity 18, member B1 | −4.50 |
| ILMN_1675401 | GTF2IP1 | General transcription factor IIi, pseudogene 1 | −2.24 |
| ILMN_1773751 | HRAS | v-Ha-ras Harvey rat sarcoma viral oncogene homolog | −1.85 |
| ILMN_1778010 | IL-32 | Interleukin 32 | −4.61 |
| ILMN_2136635 | ISCA2 | Iron-sulfur cluster assembly 2 homolog (Saccharomyces cerevisiae) | −3.40 |
| ILMN_1764861 | ISOC1 | Isochorismatase domain containing 1 | −2.40 |
| ILMN_1741599 | MEMO1 (includes EG:298787) | Mediator of cell motility 1 | −2.72 |
| ILMN_1798288 | MOB3C | MOB kinase activator 3C | −3.61 |
| ILMN_1652246 | NACAD | NAC alpha domain containing | −2.61 |
| ILMN_1758548 | NEK7 | NIMA (never in mitosis gene a)-related kinase 7 | −2.96 |
| ILMN_1806791 | OR4C46 | Olfactory receptor, family 4, subfamily C, member 46 | −4.12 |
| ILMN_1746618 | PAQR7 | Progestin and adipoQ receptor family member VII | −3.87 |
| ILMN_1707548 | RAD18 | RAD18 homolog (S. cerevisiae) | −2.44 |
| ILMN_2186597 | RPP21 | RNase P/MRP 21-kDa subunit | −3.86 |
| ILMN_1719627 | SLC27A3 | Solute carrier family 27 (fatty acid transporter), member 3 | −3.33 |
| ILMN_2400613 | SNX7 | Sorting nexin 7 | −3.33 |
| ILMN_1798827 | SRBD1 | S1 RNA binding domain 1 | −2.17 |
| ILMN_1759549 | SRGAP2 | SLIT-ROBO Rho GTPase-activating protein 2 | −2.37 |
| ILMN_1776333 | TBCEL | Tubulin-folding cofactor E-like | −3.17 |
| ILMN_2118910 | TPRKB | TP53RK binding protein | −2.29 |
| ILMN_1797384 | UROS | Uroporphyrinogen III synthase | −3.53 |
| ILMN_1803744 | VIMP | VCP-interacting membrane protein | −2.31 |
| ILMN_2212354 | WDR46 | WD repeat domain 46 | −3.00 |
| ILMN_1695362 | ZNF32 | Zinc finger protein 32 | −2.41 |
| ILMN_1738793 | ZNF71 | Zinc finger protein 71 | −7.34 |
| ILMN_1720438 | Deleted | −1.99 |
a
Six ex vivo blisters were cultured for 48 h in a 6-well plate with 50 L3 in transwells. Crawl-out LC were collected, and RNA was extracted for microarray analysis. A PALO filter (see Materials and Methods) and paired t test were used to determine genes differentially expressed in L3-exposed LC compared with unexposed LC. Normalized values from the medium-exposed blisters and values from the L3-exposed blisters were used to calculate the fold change in gene expression. The table represents the 51 genes that were significantly differentially expressed using a cutoff of 1.5-fold change in all donors.