Fig 4.

In vitro S100A15 mRNA expression in primary rat glial and meningeal cells after stimulation with bacterial supernatants or LPS. (A) Astrocytes; (B) microglia cells; (C) meningeal cells. Primary cells were stimulated with Neisseria meningitis (Nm), Pseudomonas aeruginosa (Pa), Streptococcus pneumoniae (Sp), or Staphylococcus aureus (Sa) or with lipopolysaccharide (LPS) for 6, 12, or 24 h. S100A15 mRNA expression was analyzed using SYBR green real-time RT-PCR, and results were compared to untreated sample results (c). The housekeeping gene hypoxanthin-guanin-phosphoribosyltransferase (HPRT) was used as an internal control. The experiment was performed with five animals in triplicate. Statistical significance is marked as * = P < 0.05, ** = P < 0.006, and *** = P < 0.0007 (representing the significance of the results of comparisons between control and corresponding bacterial supernatant or LPS determined using the t test).