Fig 7.

Flow cytometric analysis of blood from antibody-treated C57BL/6 mice at 24 and 48 h after intracerebral challenge with E. coli K1. The panel at 0 h represents a nondepleted and noninfected mouse. Administration of 20 μg of anti-Gr-1 MAb (RB6-8C5) led to an efficient depletion of Ly-6G⁺ Ly-6C^int granulocytes and Ly-6G⁻ Ly-6C^high inflammatory monocytes compared to the isotype control (20 μg of rat IgG2b). Mice treated with 50 μg of anti-Ly-6G MAb (1A8) showed an absence of Ly-6G⁺ Ly-6C^int granulocytes compared to the isotype control (50 μg of rat IgG_2a). Application of 10 μg of anti-CCR2 MAb (MC-21) resulted in a partial depletion of Ly-6G⁻ Ly-6C^high inflammatory monocytes compared with the isotype control (10 μg of rat IgG_2b). Data from one representative animal out of five are shown.