Fig 9.

Inflammatory cytokines and chemokines in cerebellar homogenates from anti-Gr-1-, anti-Ly-6G-, and isotype-treated mice. Levels (in pg/ml) of IL-1β (A), IL-6 (B), KC (C), and MIP-2 (D) 30 h after intracerebral E. coli K1 infection (3 × 10³ CFU/mouse) were measured by an ELISA. Each symbol represents an individual mouse. Bars indicate median values. Statistical analysis was performed by Mann-Whitney U test followed by the Bonferroni-Holm method to correct for repeated testing (*, P < 0.05; **, P < 0.01; ***, P < 0.001). The concentrations of the proinflammatory mediators were higher in depleted animals, because at this time point, the majority of immunocompetent mice had already cleared the bacteria and overcome infection (Fig. 8B and E).