Fig 3.

L. braziliensis-infected mice exhibit higher levels of IFN-γ at the infection site. L. braziliensis parasites were injected in the footpads of WT and TLR9^−/− mice and footpads were taken at peak lesion development and homogenized. RNA was isolated, cDNA was synthesized, and real-time PCR was carried out for IFN-γ (A), IL-4 (B), IL-10 (C), and IL-12p40 (D) expression. Relative mRNA expression normalized to the housekeeping HPRT gene is presented as the mean + SEM (n = at least 5 footpads per group) from 1 experiment representative of 2 independent experiment's. *, P < 0.05 (Student's t test comparing TLR9^−/− mice to control mice).