Fig 8.

Comparison of zymography profiles from serum samples from patients with confirmed leptospirosis and other infectious diseases. In panel A are shown serum samples from patients with leptospirosis at the initial phase (lanes 1 and 2) or convalescent phase (lanes 3 and 4) and sera from patients with unrelated infectious diseases: dengue (lanes 5 to 7) and malaria (lanes 8 to 10). In panel B are shown results for samples from patients with HIV (lanes 5 to 7) and Chagas' disease (lanes 8 to 10). The samples were separated by 8% SDS-PAGE with gel copolymerized with 0.1% gelatin, incubated in activation solution, and stained by Coomassie blue. The clear bands represent the regions where the substrate was digested. The arrows indicate the regions with most pronounced difference in degradation between samples from patients with leptospirosis and those from patients with other diseases.