Fig 3.
Cytokine release by BMDMs in response to C. glabrata strains. Preconfluent BMDM monolayers were infected with the matched clinical isolates DSY562 and DSY565 and with isogenic strains on the DSY562 strain background harboring either its original CgPDR1 wild-type allele (PDR1; SFY114) or the hyperactive CgPDR1L280F allele from DSY565 (L280F; SFY115). At 24 h, TNF-α (A) and IL-12 (B) levels in the supernatants were determined by ELISA. Results are means + SD of triplicate infections and are representative of three independent experiments. Differences between strains are not statistically significant (P > 0.05, one-way analysis of variance [ANOVA]). uninf, uninfected.