Adherence of C. glabrata to BMDMs. The test strains were matched clinical isolates DSY562 and DSY565 and isogenic strains on the DSY562 strain background harboring either its original CgPDR1 wild-type allele (PDR1) or the hyperactive CgPDR1L280F allele from DSY565 (L280F). (A) Quantification of competitive adherence to BMDMs. Experimental conditions were similar to those described for phagocytosis experiments in legend to Fig. 4C, with the exception that they were performed with 1 h of coincubation in medium containing 1.0 μM cytochalasin D to inhibit phagocytosis. Percent adherence ratios (PARs) represent percentages of yeasts of each strain in the total number of yeasts from both competitor strains. (B) Control PARs determined as described for competitions between the same clinical isolate labeled with the two different fluorescent proteins. (C) PARs determined as described for competitions between clinical isolate DSY565 and either cdr1Δ, pup1Δ, or cdr1Δ pup1Δ double mutant strains constructed on the same strain background. Results are means + SD of a minimum of three independent experiments performed in duplicate (with fluorescent protein labeling swapping between strains). Statistical analyses of the differences between PARs for each pair of competitor strains were performed using the Mann-Whitney U test for panel A and the unpaired Student t test for panels B and C. P values of <0.05 were considered significant, and asterisks represent P value ranges (ns, P > 0.05; *, P < 0.05; **, P < 0.01).