Fig 9.
Phagocytosis and adherence of C. glabrata to BMDMs in medium supplemented with galactose. The test strains were the isogenic strain pair on the DSY562 strain background harboring the DSY562 CgPDR1 wild-type allele (PDR1) or the hyperactive CgPDR1L280F allele from DSY565 (L280F). (A) Quantification of competitive uptake by BMDMs expressed as percent internalization ratios (PIRs; percentages of yeasts of each strain in the total number of yeasts from both competitor strains). Experimental conditions were similar to those described for Fig. 4C, with the exception that they were performed in medium supplemented with different concentrations of galactose. (B) Quantification of competitive adherence to BMDMs expressed as percent adherence ratios (PARs). Experimental conditions were similar to those described for Fig. 5A, with the exception that they were performed in medium supplemented with different concentrations of galactose. Results are means + SD of a minimum of three independent experiments performed in duplicate (with fluorescent protein labeling swapping between strains). P values of <0.05 were considered significant, and asterisks represent P value ranges (ns, P > 0.05; **, P < 0.01, Kruskal-Wallis nonparametric test, followed by Dunn's post hoc test for multiple comparisons between pairs of competitor strains).