Table 1.
Strains used in this study
| Strain | Parental strain | Plasmid used | Description or relevant genotype | Reference |
|---|---|---|---|---|
| DSY562 | Azole-susceptible clinical strain | 13 | ||
| DSY565 | Related to DSY562 | Azole-resistant clinical strain | 13 | |
| SFY114 | SFY93 | DSY562 pdr1Δ::PDR1-SAT1 | 13 | |
| SFY115 | SFY93 | DSY562 pdr1Δ::PDR1L280F-SAT1 | 13 | |
| VSY21 | DSY562 | pVS13 | ura3Δ::hph | This study |
| VSY22 | DSY565 | pVS13 | ura3Δ::hph | This study |
| VSY33 | VSY21 | pCgACU-TDH3p-GFP | DSY562 ura3Δ::hph, ScTDH3p-yEGFP(−3), URA3, CEN-ARS | This study |
| VSY38 | SFY153 | pVS13 | DSY565 cdr1Δ::FRT, ura3Δ::hph | This study |
| VSY40 | SFY155 | pVS13 | DSY565 pup1Δ::FRT, ura3Δ::hph | This study |
| VSY42 | SFY170 | pVS13 | DSY565 cdr1Δ::FRT, pup1Δ::SAT1, ura3Δ::hph | This study |
| VSY43 | SFY114 | pVS13 | DSY562 pdr1Δ::PDR1-SAT1, ura3Δ::hph | This study |
| VSY44 | SFY115 | pVS13 | DSY562 pdr1Δ::PDR1L280F-SAT1, ura3Δ::hph | This study |
| VSY55 | VSY21 | pGRB2.3 | DSY562 ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY56 | VSY22 | pGRB2.3 | DSY565 ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY57 | VSY21 | pVS19 | DSY562 ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY58 | VSY22 | pVS19 | DSY565 ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY59 | VSY21 | pVS20 | DSY562 ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY60 | VSY22 | pVS20 | DSY565 ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY65 | VSY38 | pVS20 | DSY565 cdr1Δ::FRT, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY66 | VSY40 | pVS20 | DSY565 pup1Δ::FRT, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY67 | VSY42 | pVS20 | DSY565 cdr1Δ::FRT, pup1Δ::SAT1, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY68 | VSY38 | pGRB2.3 | DSY565 cdr1Δ::FRT, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY69 | VSY40 | pGRB2.3 | DSY565 pup1Δ::FRT, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY70 | VSY42 | pGRB2.3 | DSY565 cdr1Δ::FRT, pup1Δ::SAT1, ura3Δ::hph, ScPGK1p- yEGFP, URA3, CEN-ARS | This study |
| VSY71 | VSY38 | pVS19 | DSY565 cdr1Δ::FRT, ura3Δ::hph, ScPGK1p- yEmRFP, URA3, CEN-ARS | This study |
| VSY72 | VSY40 | pVS19 | DSY565 pup1Δ::FRT, ura3Δ::hph, ScPGK1p, yEmRFP, URA3-CEN-ARS | This study |
| VSY73 | VSY42 | pVS19 | DSY565 cdr1Δ::FRT, pup1Δ::SAT1, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY101 | VSY43 | pVS20 | DSY562 pdr1Δ::PDR1-SAT1, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY102 | VSY44 | pVS20 | DSY562 pdr1Δ::PDR1L280F-SAT1, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY103 | VSY43 | pGRB2.3 | DSY562 pdr1Δ::PDR1-SAT1, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY104 | VSY44 | pGRB2.3 | DSY562 pdr1Δ::PDR1L280F-SAT1, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY105 | VSY43 | pVS19 | DSY562 pdr1Δ::PDR1-SAT1, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY106 | VSY44 | pVS19 | DSY562 pdr1Δ::PDR1L280F-SAT1, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY119 | SFY93 | pVS13 | DSY562 pdr1Δ::FRT, ura3Δ::hph | This study |
| VSY120 | SFY95 | pVS13 | DSY565 pdr1Δ::FRT, ura3Δ::hph | This study |
| VSY121 | VSY119 | pVS20 | DSY562 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY122 | VSY120 | pVS20 | DSY565 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY123 | VSY119 | pGRB2.3 | DSY562 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY124 | VSY120 | pGRB2.3 | DSY565 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY125 | VSY119 | pVS19 | DSY562 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY126 | VSY120 | pVS19 | DSY565 pdr1Δ::FRT, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY132 | SFY101 | pVS13 | DSY562 pdr1Δ::PDR1R376W-SAT1, ura3Δ::hph | This study |
| VSY133 | SFY105 | pVS13 | DSY562 pdr1Δ::PDR1T588A-SAT1, ura3Δ::hph | This study |
| VSY134 | VSY132 | pVS20 | DSY562 pdr1Δ::PDR1R376W-SAT1, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY135 | VSY133 | pVS20 | DSY562 pdr1Δ::PDR1T588A-SAT1, ura3Δ::hph, ScPGK1p, URA3, CEN-ARS | This study |
| VSY136 | VSY132 | pGRB2.3 | DSY562 pdr1Δ::PDR1R376W-SAT1, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY137 | VSY133 | pGRB2.3 | DSY562 pdr1Δ::PDR1T588A-SAT1, ura3Δ::hph, ScPGK1p-yEGFP, URA3, CEN-ARS | This study |
| VSY138 | VSY132 | pVS19 | DSY562 pdr1Δ::PDR1R376W-SAT1, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |
| VSY139 | VSY133 | pVS19 | DSY562 pdr1Δ::PDR1T588A-SAT1, ura3Δ::hph, ScPGK1p-yEmRFP, URA3, CEN-ARS | This study |