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Agglutination assay for direct visualization of bacterium–gal-3 interaction. Gal-3 or PBS alone (negative control) was incubated with B. longum subsp. infantis ATCC 15702. Gal-3 exposure resulted in agglutination of the bacteria, as evidence by the absence of a dense pellet of bacteria in the bottom of the well (A). PBS alone did not agglutinate the bacteria, resulting in pellet formation at the bottom of the well (B).
