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. 2013 Jun;79(11):3507–3510. doi: 10.1128/AEM.03694-12

Fig 5.

Fig 5

(A) Binding of TAMRA-labeled bacteria to recombinant gal-3 in a solid-phase binding assay. B. longum subsp. infantis ATCC 15702 and ATCC 15697 and E. coli O157:H7 NCTC 12900 were incubated in gal-3-coated wells for 1 h. (B) Binding of TAMRA-labeled B. longum subsp. infantis ATCC 15702 to recombinant gal-3 in a solid-phase binding assay. TAMRA-labeled bacteria were incubated in gal-3-coated wells (white bars) or in gal-3-coated wells in the presence of lactose (gray bars). (C) Binding of TAMRA-labeled B. longum subsp. infantis ATCC 15702 to recombinant gal-3 in a solid-phase binding assay. Bacteria were incubated in gal-3-coated wells (white) or in gal-3-coated wells in the presence of the soluble gal-3 CRD (gal-3C; gray) or full-length soluble gal-3 (black). All experiments were carried out in triplicate (n = 3). The data are presented as means ± the standard deviations. *, P < 0.05; **, P < 0.01; AU, arbitrary units.