Fig 5.

PCR identification of Lactococcus lactis. PCR amplification products in lanes 2 to 8 were determined using primers CreF and LacreR. Lane 1, marker λDNA/HindIII; lane 2, no-DNA control; lane 3, L. lactis subsp. cremoris LMG 6897^T; lane 4, M78; lane 5, M104; lane 6, L. lactis subsp. lactis LMG 6890^T; lane 7, L. lactis subsp. lactis F1 Nis⁺ strain; lane 8, L. lactis subsp. lactis NCFB 1402 Nis⁺ strain. PCR amplification products in lanes 10 to 16 were determined using primers LacF and LacreR. Lane 9, marker λDNA/HindIII; lane 10, no-DNA control; lane 11, L. lactis subsp. cremoris LMG 6897^T; lane 12, M78; lane 13, M104; lane 14, L. lactis subsp. lactis LMG 6890^T; lane 15, L. lactis subsp. lactis F1 Nis⁺ strain; lane 16, L. lactis subsp. lactis NCFB 1402 Nis⁺ strain. The compact/solid square indicates the presence of amplified PCR product bands for the L. lactis subsp. cremoris LMG 6897^T strain and the wild Nis⁺ M78 and M104 isolates when the cremoris-specific primer pair CreF and LacreR was used, whereas the dashed square indicates the absence of amplified PCR product bands for the same strains when the subspecies lactis-specific primer pair LacF and LacreR was used.