Table 2.
Differentiating phenotypic/biochemical identification reactions of the wild Nis+ Lactococcus lactis strains M78 and M104 in comparison with reference strainsa
| Phenotypic test |
Lactococcus lactis strain |
|||||
|---|---|---|---|---|---|---|
| M78 | M104 | LMG 6890T | LMG 6897T | NCFB 1402 | F1 | |
| NH3 from arginine | + | + | + | − | + | + |
| Growth in 4% salt | + | + | + | − | + | + |
| Fermentation of: | ||||||
| d-Ribose | + | + | + | − | + | + |
| d-Xylose | + | + | + | − | − | − |
| d-Galactose | + | + | + | − | + | + |
| d-Mannitol | + | + | − | − | + | + |
| Amygdalin | + | + | − | − | − | − |
| Arbutin | + | + | + | − | (+) | (+) |
| Esculin | + | + | + | − | + | + |
| Salicin | + | + | + | − | + | + |
| d-Cellobiose | + | + | + | − | + | + |
| d-Maltose | + | + | + | − | + | − |
| d-Lactose | + | + | + | + | + | − |
| d-Sucrose | + | + | − | − | + | + |
| d-Trehalose | + | + | + | − | − | − |
| Amidon (starch) | (+)d | (+)d | − | − | − | − |
| Gentiobiose | + | + | − | − | + | + |
All strains were Gram-positive, catalase-negative, homofermentative cocci which grew at 15°C. None grew at 45°C, in 6.5% salt, and on kanamycin esculin azide (KAA) agar. None survived heating at 60°C for 30 min. All strains fermented d-glucose, d-fructose, d-mannose, and N-acetylglucosamine. None fermented glycerol, erythritol, d-arabinose, l-arabinose, l-xylose, adonitol, α-methyl-d-xylopyranoside, l-sorbose, l-rhamnose, dulcitol, inositol, d-sorbitol, α-methyl-d-mannopyranoside, α-methyl-d-glucopyranoside, d-melibiose, inulin, d-melezitose, d-raffinose, glycogen, xylitol, d-turanose, d-lyxose, d-tagatose, d-fucose, l-fucose, d-arabitol, l-arabitol, gluconate, 2-ketogluconate, or 5-ketogluconate. +, positive reaction; −, negative reaction; (+), weak reaction; (+)d, delayed reaction.