Table 3.
Enzymatic activity reactions of the wild Nis+ Lactococcus lactis M78 and M104 in comparison with reference strains determined by the API ZYM methoda
| Enzyme assayed for |
Lactococcus lactis strain reaction color grade |
|||||
|---|---|---|---|---|---|---|
| M78 | M104 | LMG 6890T | LMG 6897T | NCFB 1402 | F1 | |
| Alkaline phosphatase | 5 | 5 | 5 | 3 | 4 | 4 |
| Esterase (C4) | 3 | 3 | 3 | 4 | 4 | 4 |
| Esterase lipase (C8) | 4 | 4 | 4 | 5 | 4 | 4 |
| Lipase (C14) | − | − | − | − | − | − |
| Leucine arylamidase | 5 | 5 | 5 | 5 | 5 | 5 |
| Valine arylamidase | 4 | 4 | 4 | 2 | 4 | 4 |
| Cystine arylamidase | 4 | 4 | 4 | 2 | 4 | 4 |
| Trypsin | − | − | − | − | − | − |
| α-Chymotrypsin | 3 | 3 | 2 | 3 | 3 | 3 |
| Acid phosphatase | 5 | 5 | 5 | 5 | 5 | 5 |
| Naphthol-AS-BI-phosphohydrolase | 5 | 5 | 5 | 5 | 5 | 5 |
| α-Galactosidase | − | − | − | − | − | − |
| β-Galactosidase | − | − | − | − | − | − |
| β-Glucuronidase | − | − | − | − | − | − |
| α-Glucosidase | 5 | 5 | 5 | − | − | − |
| β-Glucosidase | − | − | − | − | − | − |
| N-Acetyl-β-glucosaminidase | − | − | − | − | − | − |
| α-Mannosidase | − | − | − | − | − | − |
| α-Fucosidase | − | − | − | − | − | − |
a
Numbers indicate a positive reaction based on the change in color intensity in each API ZYM cupule. According to the manufacturer's instructions, the color change was graded using a scale from 1 (extremely weak reaction) to 5 (very strong positive reaction). −, negative reaction similar to that seen with the control. Reactions with a grade of 3 to 5 are considered as clearly positive.