Table 3.
Sample ID |
Bacteria |
Archaea |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of qualified reads | OTU count | No. of observed species | Chao1 index | Shannon index | Simpson indexb | No. of qualified reads | OTU count | No. of observed species | Chao1 index | Shannon index | Simpson indexb | |
DIS-NDW | 22,309 | 2,031 | 2,024.000 | 2,759.10 | 7.68 | 0.971 | 3,757 | 102 | 101.900 | 125.906 | 3.500 | 0.828 |
DIS-BWI | 23,515 | 1,828 | 1,827.500 | 2,673.23 | 6.67 | 0.957 | 357 | 126 | 72.400 | 95.796 | 4.718 | 0.911 |
DIS-LCL | 19,545 | 2,297 | 2,295.500 | 3,080.16 | 8.53 | 0.989 | 1,359 | 110 | 109.900 | 219.150 | 5.083 | 0.937 |
ATL-NDW | 18,787 | 1,816 | 1,808.300 | 2,643.19 | 7.93 | 0.981 | 3,299 | 97 | 97.000 | 171.000 | 3.654 | 0.853 |
ATL-BWI | 22,578 | 2,117 | 2,116.700 | 3,095.92 | 7.93 | 0.985 | 7,356 | 130 | 130.000 | 164.731 | 3.269 | 0.798 |
ATL-UCL1 | 18,925 | 1,959 | 1,953.500 | 2,664.68 | 8.33 | 0.990 | 955 | 93 | 92.900 | 146.564 | 5.065 | 0.944 |
ATL-UCL2 | 16,737 | 2,169 | 2,166.800 | 2,994.93 | 8.40 | 0.987 | 1,145 | 101 | 100.700 | 151.184 | 4.862 | 0.936 |
ATL-LCL | 20,474 | 2,299 | 2,298.700 | 2,934.74 | 8.38 | 0.985 | 639 | 71 | 70.000 | 109.985 | 4.648 | 0.932 |
Samples were rarefied at the smallest libraries, and results were averaged over 100 repetitions. In Atlantis II, all metrics more or less agree that bacteria and archaea have higher diversity in transition zones and the brine layers than in normal deep water. In Discovery, the LCL was consistently the highest in diversity for bacteria and archaea, followed by NDW and then BWI (with some differences between species richness and diversity indices). The number of unclassified reads was 10,600.
The Simpson index used here is the Gini-Simpson index.