Table 2.
Primer | Sequence |
---|---|
cidA-pro-F | 5′-CCCGCATGCAGCAAATTATCAATGATGAAGTAGATA-3′ |
cidA-pro-R | 5′-CCCGGATCCCGCCATCCCTTTCTAAATAC-3′ |
lrgA-pro-F | 5′-CCCGCATGCCGATAAAATTCACATGTTAAAGC-3′ |
lrgA-pro-R | 5′-CCCGGATCCCGTTTGATTTAACTAAAGTATAGATGG-3′ |
ldh1-pro-F | 5′-CCCGCATGCATGGCTTTTAATAAATTTTC-3′ |
ldh1-pro-R | 5′-CCCGGATCCTACAAAAACTCCCTTATGAT-3′ |
cidA-rt-F | 5′-GGGTAGAAGACGGTGCAAAC-3′ |
cidA-rt-R | 5′-TTTAGCGTAATTTCGGAAGCA-3′ |
lrgA-rt-F | 5′-GCATCAAAACCAGCACACTTT-3′ |
lrgA-rt-R | 5′-TGATGCAGGCATAGGAATTG-3′ |
sigA-rt-F | 5′-AACTGAATCCAAGTCATCTTAGTC-3′ |
sigA-rt-R | 5′-TCATCACCTTGTTCAATACGTTTG-3′ |
DsRed-F | 5′-CAGAGTCGACTGATTAACTTTATAAGGAGGAAATACATATGGACAACACCGAGG-3′ |
DsRed-R | 5′-ACATGCATGCTACAGGAACAGGTGGTGGCGG-3′ |
sGFP-F | 5′-CACGAATTCTGATTAACTTTATAAGGAGGAAAAACATATGCCCGGGAGCAAAGGAG-3′ |
sGFP-R | 5′-CCTGGCGCGCCTTCTTATTTGTAGAGCTCATCCATGCC-3′ |
lrgA-R | 5′-GCTGGATCCACGTTTGATTTAACTAAAGTATAGATGGCTCAC-3′ |
cidA-R | 5′-GCCGGAATTCTAAATACGTCTAAATTGTTACAATAACTATTATAAAGATGGCG-3′ |
cidABC-lrgAB-F | 5′-GTTTCCAGTCCATTCAAGCGTTCCGCACATGACCAATACGCAGTACAG-3′ |
lrgAB-cidABC-F | 5′-CTGTACTGCGTATTGGTCATGTGCGGAACGCTTGAATGGACTGGAAAC-3′ |
sDsRed-F | 5′-AGCGGATCCAGATAATCTATAAAAGGAGG-3′ |
sDsRed-R | 5′-TCTTGCATGCTTATAAAAACAAATGATGACGAC-3′ |
Recognition sequences for restriction endonucleases used for cloning are underlined.