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. 2013 Jun;79(11):3413–3424. doi: 10.1128/AEM.00395-13

Table 2.

Primers used in this studya

Primer Sequence
cidA-pro-F 5′-CCCGCATGCAGCAAATTATCAATGATGAAGTAGATA-3′
cidA-pro-R 5′-CCCGGATCCCGCCATCCCTTTCTAAATAC-3′
lrgA-pro-F 5′-CCCGCATGCCGATAAAATTCACATGTTAAAGC-3′
lrgA-pro-R 5′-CCCGGATCCCGTTTGATTTAACTAAAGTATAGATGG-3′
ldh1-pro-F 5′-CCCGCATGCATGGCTTTTAATAAATTTTC-3′
ldh1-pro-R 5′-CCCGGATCCTACAAAAACTCCCTTATGAT-3′
cidA-rt-F 5′-GGGTAGAAGACGGTGCAAAC-3′
cidA-rt-R 5′-TTTAGCGTAATTTCGGAAGCA-3′
lrgA-rt-F 5′-GCATCAAAACCAGCACACTTT-3′
lrgA-rt-R 5′-TGATGCAGGCATAGGAATTG-3′
sigA-rt-F 5′-AACTGAATCCAAGTCATCTTAGTC-3′
sigA-rt-R 5′-TCATCACCTTGTTCAATACGTTTG-3′
DsRed-F 5′-CAGAGTCGACTGATTAACTTTATAAGGAGGAAATACATATGGACAACACCGAGG-3′
DsRed-R 5′-ACATGCATGCTACAGGAACAGGTGGTGGCGG-3′
sGFP-F 5′-CACGAATTCTGATTAACTTTATAAGGAGGAAAAACATATGCCCGGGAGCAAAGGAG-3′
sGFP-R 5′-CCTGGCGCGCCTTCTTATTTGTAGAGCTCATCCATGCC-3′
lrgA-R 5′-GCTGGATCCACGTTTGATTTAACTAAAGTATAGATGGCTCAC-3′
cidA-R 5′-GCCGGAATTCTAAATACGTCTAAATTGTTACAATAACTATTATAAAGATGGCG-3′
cidABC-lrgAB-F 5′-GTTTCCAGTCCATTCAAGCGTTCCGCACATGACCAATACGCAGTACAG-3′
lrgAB-cidABC-F 5′-CTGTACTGCGTATTGGTCATGTGCGGAACGCTTGAATGGACTGGAAAC-3′
sDsRed-F 5′-AGCGGATCCAGATAATCTATAAAAGGAGG-3′
sDsRed-R 5′-TCTTGCATGCTTATAAAAACAAATGATGACGAC-3′
a

Recognition sequences for restriction endonucleases used for cloning are underlined.