Table 3.

Multilocus genotyping of T. gondii isolates in bats from Myanmar by PCR-RFLP analysis

Host and isolate ID	PCR-RFLP genotype by genetic markera											ToxoDB genotyped
	SAG1	5′ + 3′ SAG2b	alter.SAG2	SAG3	BTUB	GRA6	c22-8	c29-2c	L358	PK1	Apico
Miniopterus fuliginosus
TgBatMm1	ND	II	I	I	ND	I	I	u-1	II	I	I	Novel 1
TgBatMm2	ND	I	I	I	I	ND	I	u-1	II	I	I	Novel 2
TgBatMm3	II/III	I	I	I	I	ND	ND	u-1	ND	I	I	Novel 3
TgBatMm4	II/III	I	I	I	ND	I	I	u-1	II	II	I	Novel 4
TgBatMm5	ND	I	I	I	I	I	I	u-1	I+II	I	I	Novel 5
TgBatMm6, -9	I	I	I	I	I	I	I	u-1	I	I	I	Novel 6
TgBatMm7	I	I	I	I	I	I	I	u-1	ND	I	I	Novel 7
TgBatMm8	II/III	I	I	ND	I	I	I	u-1	II	I	I	Novel 8
TgBatMm10	ND	I	I	I	I	III	I	I	u-1	ND	I	Novel 9
TgBatMm11	I	I	I	I	I	I	I	I	I	I	I	10
TgBatMm12	I	I	ND	I	I	III	I	I	II	ND	I	Novel 10
Megaderma lyra
TgBatMm13	I	I	I	I	I	III	I	ND	I	I	I	Novel 11
TgBatMm14	I	I	I	I	I	I	I	u-1	II	ND	I	Novel 12
Myotis chinensis
TgBatMm15	I	I	I	I	I	I	I	I	ND	I	I	10 (?)
TgBatMm16	I	I	I	I	I	I	I	ND	II	I	I	Novel 13
TgBatMm17	I	I	I	I	I	III	I	I	II	I	ND	Novel 14
TgBatMm18	I	I	I	I	I	I	I	ND	I	I	I	10, 27 (?)
TgBatMm19	I	I	I	I	I	I	I	I	I	ND	ND	10 (?)

^aGenotypes of T. gondii were determined according to PCR-RFLP analysis of 12 genetic loci, each locus usually producing three different genotypes, which were grouped into types I, II, or III, based on the three clonal types of reference strains (ToxoDB 10, 1, and 2). ND, no amplification detected due to a low DNA concentration in the sample.

^bSAG2 marker based on the 5′ and 3′ ends of the gene sequence.

^cu-1 is the new allele different from the clonal type I, II, and III alleles.

^dA question mark indicates that the genotype of T. gondii in the sample may represent “others” that are not listed in ToxoDB due to some missing genetic markers.