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Kinetic constants for hydrolysis of p-nitrophenol-β-d-glucopyranoside
| TnBgl1A variant | Vmaxa (μmol min−1 mg−1) | kcata (s−1) | Kma (mM) | kcat/Kma (s−1 mM−1) |
|---|---|---|---|---|
| wt | 179 ± 4 | 154 ± 3 | 0.45 ± 0.05 | 342 ± 39 |
| V168S | 204 ± 7 | 175 ± 6 | 0.20 ± 0.02 | 875 ± 80 |
| N220F | 732 ± 30 | 628 ± 25 | 1.28 ± 0.16 | 491 ± 64 |
| G222F | 191 ± 6 | 164 ± 5 | 0.77 ± 0.09 | 213 ± 26 |
| W322F | 87 ± 4 | 74 ± 3 | 0.17 ± 0.02 | 452 ± 58 |
Data are presented as averages with ranges representing 95% confidence intervals.
