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Total activity, rs/rh, and expected yield for the selected mutants
| TnBgl1A variant | Total initial reaction ratea (μmol min−1 mg−1) | rs/rha | Expected yield (%) |
|---|---|---|---|
| wt | 285 ± 21 | 0.20 ± 0.03 | 17 |
| V168S | 262 ± 18 | 0.20 ± 0.02 | 17 |
| N220F | 163 ± 11 | 1.38 ± 0.20 | 58 |
| G222F | 199 ± 16 | 0.22 ± 0.03 | 18 |
| W322F | 141 ± 11 | 0.12 ± 0.08 | 11 |
Data are presented as averages with ranges representing 95% confidence intervals.
