Fig 2.

Hyperactivation of NMD blunts stress-induced autophagy. (A and B) U2OS cells stably expressing a GFP-LC3 fusion were treated with rapamycin (A) or tunicamycin (B) for the indicated time periods and immunoblotted for GFP and other noted proteins. (C and D) These cells (C) and MEFs (D) expressing GFP-LC3 were treated with rapamycin for 8 h or deprived of amino acids for 36 h, and LC3 foci were quantitated. *, P < 0.05 by Student's t test compared to a similarly treated control. (E) U2OS cells expressing GFP-LC3 were treated with rapamycin with and without chloroquine and immunoblotted for GFP and other noted proteins.