Fig 6.

FXR inhibits the expression of glucose-induced genes. Genes identified as being regulated by both glucose and FXR using microarray analysis were analyzed for mRNA expression in IHH incubated for 24 h in a medium containing low (1 mM) or high (11 mM) glucose concentrations and vehicle (DMSO) or GW4064 (5 μM). HK3, hexokinase 3; PGM1, phosphoglucomutase 1; TPI1, triosephosphate isomerase 1; PGAM1, phosphoglycerate mutase 1; ACSS1, acyl-CoA synthetase short-chain family member 1; LDHA, lactate dehydrogenase A. Gene and control 36B4 mRNA levels were measured by real-time quantitative PCR. The values are expressed relative to those at low glucose concentration with vehicle, which were arbitrarily set to 1.