Skip to main content
. 2013 Jun;33(11):2163–2177. doi: 10.1128/MCB.01019-12

Fig 5.

Fig 5

Arkadia interacts with sumoylated PML through its SIMs. (A and B) Arkadia specifically interacts with SUMO-modified PML through its SIMs. HEK293 cells were transfected with the indicated Flag-Ark constructs together with HA-PML (A) or with GFP-PML-wt or GFP-PML-3KR mutated in the 3 SUMO consensus sites (B). Cells were treated or not with arsenic for 1 h. Flag immunoprecipates were analyzed by Western blotting using anti-Flag and anti-HA (A) or anti-GFP (B) antibody. The corresponding whole-cell lysates (input) were analyzed using anti-HA (A) or anti-GFP (B) antibody. #, nonspecific binding of unmodified GFP-PML under all conditions. Numbers to the left of the gels are molecular masses (in kDa). (C) Arkadia specifically interacts with SUMO-modified PML-RARα through its SIMs. HEK293 cells were transfected with Flag-Ark-wt or Flag-Ark-SIM123* together with PML-RARα. Cells were treated or not with arsenic for 1 h. Flag immunoprecipates were analyzed by Western blotting using anti-Flag and anti-PML antibodies. The corresponding whole-cell lysates (input) were analyzed using anti-PML antibodies.