Fig 1.

Productive infection of cultured primary adult murine TG neurons with HSV-2 LAT region deletion and chimeric viruses (multiplicity of infection [MOI], 30; 10 h postinoculation). (A) Percentages of total (hatched bars), A5+ (black bars), and KH10+ (gray bars) neurons productively infected with HSV-1 and HSV-2, as identified by MAbs A5 and KH10, and polyclonal antisera against HSV-1 or HSV-2. (These data were published previously [3] and are shown here for reference). (B) Percentages of A5+ and KH10+ neurons productively infected with HSV-2 wild type (333) and HSV-2 LAT region deletion viruses (dLAT and LAP2). (C) Percentages of A5+ and KH10+ neurons productively infected with HSV-2 wild type (333), chimeric virus HSV2/LAT1, and its rescuant, HSV2/LAT1-R. (D) Maps illustrating HSV-2 LAT region deletion and chimeric sequence swap. Deletions are indicated by white boxes on the HSV-2 LAT region (black), and the HSV-1 sequence placed into HSV-2 is indicated by a hatched box on the HSV-2 background (black). ICP0 transcript is shown for reference.