Fig 2.
Mutational effects on cap N7-MTase activity of nsp14 in vitro. (A and B) TLC analysis of nuclease P1-resistant cap structures released from 32P-labeled G*pppA-RNA methylated by nsp14 mutants. The markers m7G*pppA and G*pppA were prepared with commercial vaccinia virus capping enzymes. The positions of origin and migration of m7G*pppA and G*pppA are indicated on the left. The ratios of conversion of G*pppA to m7G*pppA are show at the bottom. (C) GpppA-RNA was used to test the methylation activities of nsp14 mutants (n = 3; mean values ± SD are shown).