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Subcellular distribution of wild-type E3/19K and E3/19K mutants as analyzed by immunofluorescence. Transfected 293 cells stably expressing wild-type E3/19K (EEE), EEE*, EEK, EKK, EKE*, or EKE were stained for immunofluorescence microscopy using MAb 3A9 followed by Cy3-conjugated goat anti-mouse IgG. Cells were either left intact for the assessment of expression on the cell surface (A to F) or permeabilized with Triton X-100 for internal staining (G to L).
