Fig 6.

Differential transport of HLA molecules in cells expressing E3/19K chimeras. Transport of HLA molecules was analyzed in a pulse-chase experiment (20-min pulse, 2.5-h chase). First, HLA-A2 was immunoprecipitated with MAb BB7.2 (A). The remaining HLA-A, -B, and -C molecules were precipitated with MAb W6/32 (B). Precipitated material was treated with endo H to enable differentiation between molecules transported through the medial Golgi apparatus, where they acquire endo H-resistant (^R) complex-type carbohydrates, and those that still contain high-mannose carbohydrates that are cleaved by endo H (^c) and therefore have not reached this compartment. The percentage of HLA molecules that have acquired endo H resistance (% endo H resistant) relative to the total amount of HLA precipitated is shown separately in corresponding bar diagrams for HLA-A2 (A) and the remaining HLA molecules (B).