Fig 7.

Neuropathological lesions in the brain of macaque GC98. Histological staining, immunohistochemistry, and double-label in situ hybridization staining of brain sections were carried out. (A) Vacuolar leukoencephalopathy (hematoxylin and eosin staining; cerebellum); (B) vacuolar change with associated infected macrophages (SIV ISH and IHC for the macrophage marker Iba-1; cerebellum); (C) representative perivascular lesion of infected macrophages devoid of MNGCs (hematoxylin and eosin staining; frontal cortex); (D) perivascular lesion of infected macrophages (SIV ISH and IHC for Iba-1; frontal cortex); (E and F) ramified microglia (SIV ISH and IHC for Iba-1; thalamus).