Fig 8.

Griffithsin does not induce DC maturation or induce apoptosis. (A) Immature moDCs were exposed to medium (mock) or LPS (10 μg/ml) for 24 h at 37°C, and then one set each was stimulated with LPS (10 μg/ml) for 1 h. The cells were then analyzed by flow cytometry for surface marker CD83 expression. Data are mean percentages of CD83⁺ + SEM from six (24 h) and two (+LPS) independent experiments. (B) Immature moDCs were exposed to the indicated concentrations of GRFT overnight and analyzed by flow cytometry for percentage of cells that are annexin V⁺/7AAD⁻ (early apoptosis) and annexin V⁺/7AAD⁺ (late apoptosis). Data are means + SEM from two independent experiments. Asterisks indicate significance compared to mock-treated cells under each condition (P < 0.05, ANOVA).