Fig 1.

Membrane curvature or particle formation is not required for uropod localization. (A) An illustration indicating the virus assembly step that is inhibited by the CA mutations P99A and EE75,76AA. (B) P2 cells expressing P99A/Gag-YFP and EE75,76AA/Gag-YFP were examined for virus release efficiency as described in Materials and Methods. (C) Quantification of virus release efficiency. Data from three different experiments are shown as means ± standard deviations. (D) P2 cells were infected with VSV-G-pseudotyped viruses encoding P99A/Gag-YFP (upper panel) or EE75,76AA/Gag-YFP (lower panel) and immunostained for PSGL-1 before fixation. Uropod localization of Gag-YFP was assessed using PSGL-1 as a uropod marker. (E) Gag polarity index values for wild-type Gag-YFP, P99A/Gag-YFP, and EE75,76AA/Gag-YFP in polarized P2 cells were determined as described in Materials and Methods. n, the number of cells used for quantification. Error bars represent standard errors of the means. ns, not significant.