Fig 6.

Membrane curvature is not required for specific UDM association. (A to C) P2 cells were infected with VSV-G-pseudotyped virus particles encoding the budding-deficient mutants P99A/Gag-YFP (top rows) and EE75,76AA/Gag-YFP (bottom rows). These cells were immunostained for PSGL-1 (A), CD43 (B), or CD59 (C) prior to fixation. Maximum projections reconstructed from z-stack images of cells are shown. (D) Copatching between Gag and uropod markers was quantified with the square of Pearson's correlation coefficient (R²). n, the number of cells used for quantification. Error bars represent standard errors of the means. P values were calculated based on comparisons between Gag copatching with PSGL-1 and other uropod markers. ***, P < 0.001; ns, not significant.