Fig 5.

The viral protein NSP3 alters the nucleocytoplasmic distribution of cellular mRNAs in rotavirus-infected cells. MA104 cells were transfected with the indicated siRNAs; 40 8 h posttransfection, cells were either infected (+virus) or mock infected (-virus), and 12 hpi, cells were harvested, and nuclear (Nuc) and cytoplasmic (Cytop) fractions were obtained as described in Materials and Methods. Total RNA was extracted with TRIzol, and grp78, grp94, and GAPDH mRNAs were quantitated in each fraction by qRT-PCR. The qRT-PCR results are expressed as a fold increase relative to the amount of each mRNA (grp78, grp94, or GAPDH) present in the cytoplasmic fraction of mock-infected cells, transfected with the control siRNA, which was taken as 1. The arithmetic mean ± SEM for three independent experiments performed in triplicate is shown. Asterisks indicate significant differences (P < 0.05) between treated cells and their mock counterparts, determined by ANOVA.