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Phosphorylated Y132 of M1 is essential for nuclear import of M1. (A) The localization of WT and mutated M1s (Y132A/F/D) was determined in a 12-plasmid reverse genetic system. At 24, 36, and 48 h p.t., transfected cells were fixed and detected by anti-M1 antibody (green). (B) M1s (WT and Y132A mutant) were detected by an anti-M1 monoclonal antibody (green) at different time points during virus recuse. Bars, 10 μm. The nucleus was stained with DAPI (4′,6-diamidino-2-phenylindole; blue).
