Fig 8.

Hsp90β knockdown blocks nuclear transport of BALF5 DNA polymerase catalytic subunit. (A) AGS-CR2/EGFP-EBV cells expressing either luciferase shRNA (shLuc) (a) or Hsp90β shRNA (shHsp90β) (b) were subjected to immunofluorescence analysis. Each cell was transfected with pcDNA-BZLF1 to induce lytic replication and harvested 24 h posttransfection. Harvested cells were fixed and immunostained with anti-BALF5 (green), anti-Hsp90β (red), and anti-BMRF1 (yellow) antibodies. Nuclei were stained with DAPI (blue). (B) HeLa cells expressing either luciferase shRNA (shLuc) (a) or Hsp90β shRNA (shHsp90β) (b) were transfected with pcDNA-FLAG-BALF5 and pcDNA-BMRF1. At 24 h posttransfection, cells were fixed and immunostained with anti-FLAG (green), anti-Hsp90β (red), and anti-BMRF1 (yellow) antibodies. Nuclei were stained with DAPI (blue). Hsp90β expression levels of each cell were confirmed by immunoblot analysis (c).