Fig 4.
Inhibition of the stress response had no effect on the apoptosis or autophagy pathways. Lysates of scrambled siRNA (scramble)- and PKR siRNA (PKRKD)-transfected HeLa cells infected with mengo-wt or mengo-Zn (MOI = 10) were analyzed for expression of PARP, PKR, LC3, and actin by Western blotting. PKR knockdown, which prevents SG formation, had no effect on the induction of apoptosis as detected by the degradation of full-length (FL) PARP into the smaller cleavage product (indicated by the asterisk). Additionally, lack of SG formation had no effect on the activation of the autophagy pathway as determined by the conversion of LC3 I into LC3 II.