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RCC1-GST is catalytically active. The fluorescence of nucleotide analog mant-GDP (N-methyl-anthraniloyl-tagged GDP; λex = 355 nm, λem = 420 to 460 nm) was measured by spectrofluorimetry after 15 min. Reactions were performed at 25°C with His-Ran, GST, or GST-L (1.5 μm each). The analogue was at 50× molar excess (75 μm). The reactions were repeated in the presence of RCC1-GST (30 nM) or a combination of RCC1-GST (30 nM) and GST-L (1.5 μm).
