Fig 6.

Comparison of the absorbance values of the PMTV mutant deficient in 8K and DI RNA production. (A) Levels of PMTV virion accumulation detected by double-antibody sandwich ELISA as indicated by absorbance values at 405 nm. Plant extracts were prepared from upper leaves at 14 dpi. The average absorbance of a healthy plant extract was equal to 0.19. Asterisks indicate that the absorbance values were significantly different (P < 0.05, Student t test) from the wt. ELISAs were conducted three times (n = 18). (B) Effect of the lack/presence of 8K and DI on virus accumulation. Accumulation of wt progeny was considered to be 100%. The data were collected from three independent experiments (n = 18). Asterisks indicate that the absorbance values were significantly different (P < 0.05, Student t test) from the wt. The RNA-TGB mutants are also presented in the table (C). An upward arrow indicates statistically significant increase in virus accumulation compared to the wt. A downward arrow indicates statistically significant reduction in virus accumulation compared to the wt. Double-headed arrow, no change; ns, not significant (P > 0.05, Student t test).