Fig 5.

HCMV ΔUL37x1 Mut does not recruit Bax to MAM lipid rafts. HFFs were infected with the HCMV ΔUL37x1 Mut (BADsubUL37x1) at an MOI of 1.5. DRMs were harvested from the cells at early (24 hpi), late (72 and 96 hpi), and very late (120 hpi) times of infection and purified as in Fig. 4 and as described previously (41). Each flotation gradient fraction (30 μl) was resolved by SDS-PAGE and analyzed by Western blotting using anti-Bax (1:500; Millipore) or mouse anti-Cav1 (1:500; BD Biosciences) antibodies.