Table 1.

Quantitative real-time RT-PCR primers and probes

RNA target	Primer or probea	Sequenceb	Accession no.c
Chicken IFN-α	ChIFNα F	5′-GAC AGC CAA CGC CAA AGC-3′	U07868
	ChIFNα R	5′-GTC GCT GCT GTC CAA GCA TT-3′
	ChIFNα P	5′-(FAM)-CTC AAC CGG ATC CAC CGC TAC ACC-(TAMRA)-3′
Turkey IFN-α	TkIFNα F	5′-GAC AGC CAA CGC CAA AGC-3′	U28140
	TkIFNα R	5′-GTG GCT GTC CGC CAA GCA TT-3′
	TkIFNα P	5′-(FAM)-CTC AAC CAG ATC CAG CGG TAC GCC-(TAMRA)-3′

^aF, forward primer; R, reverse primer; P, probe.

^bFAM, 6-carboxyfluorescein; TAMRA, carboxytetramethylrhodamine.

^cRefers to genomic DNA sequence.