Fig 1.

One-step growth kinetics of HAdV-5.polF421Y is equivalent to that of the wild type. 293β5 cells were infected with wild-type Ad5.eGFP or HAdV-5.polF421Y. Infectious titers of combined cell lysate and supernatant were determined at the indicated times postinfection by flow cytometry for eGFP expression of serially infected 293β5 cells. Data are the average of two titer determinations from one time course experiment ± standard deviations (SD). The limit of detection is indicated by the horizontal dotted line.