Fig 2.
HAdV-5.polF421Y evolved resistance to 9C12 neutralizing antibody. The 9C12 IC50s were determined on 293β5 cells of lysates from cells infected with HAdV-5.polF421Y after the indicated rounds of 9C12 selection (A); purified HAdV-5.polF421Y, a plaque-purified HAdV-5.polF421Y bearing the G434D hexon mutation (9R-01), and wild-type vector engineered with the G434D mutation (HAdV-5.hexonG434D) (B); or lysates from cells infected with wild-type Ad5.eGFP after the indicated rounds of 9C12 selection (C). Data are the mean percentages of control infection from three independent experiments ± SD.