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. 2013 May;87(10):6037–6043. doi: 10.1128/JVI.00153-13

Fig 2.

Fig 2

Levels of miR-145 decrease upon differentiation of HPV-31-positive cells. (A) E7 protein mediates repression of miR-145. Shown is the qPCR analysis of miR-145 levels in monolayer cultures of viv, viv-31gen, viv-31E6, and viv-31E7 cells. The data are normalized to U6 levels and are represented as fold difference from miR-145 levels in normal keratinocytes (viv), which were set to 1.0. (B) qPCR analysis of miR-145 levels in normal keratinocytes induced to differentiate in high calcium media. The data are normalized to U6 levels and are represented as fold change relative to miR-145 levels in undifferentiated cultures (set to 1.0). (C) qPCR analysis of miR-145 levels in cells stably maintaining HPV-31 episome (viv-31gen) monolayer cultures upon differentiation in high calcium. The data are normalized to U6 levels and are represented as fold change from levels seen in undifferentiated cells. Results are averages from three independent experiments, and standard errors are shown. ca, calcium-induced differentiation.