Fig 1.

HSV-2 UL21 is expressed as a late gene. Vero cells were infected at an MOI of 5.0 with HSV-2 WT in the absence or presence of 400 ng/ml PAA. At the indicated times postinfection, total cell lysates were collected, electrophoresed through 10% polyacrylamide gels, and transferred onto PVDF membranes. Membranes were probed with the antisera indicated on the right. The UL21 gene of HSV-2 encodes a 532-amino-acid protein with a predicted mass of 58 kDa. Molecular mass markers in kDa are shown on the left.