Fig 4.

Solution structure of MNV VPg 11-85. (A) Backbone trace of the 20 lowest-energy conformers of MNV VPg 11-85, calculated by ARIA (62) and CNS (60). The side chain of the nucleotide-accepting tyrosine from one of the conformers is indicated. (B) Representative conformer of MNV VPg 11-85. The N and C termini are indicated. (C) Overlay of the structured cores of FCV VPg (tan) and MNV VPg (green); the backbone RMSD between the two structures is ∼2 Å. (D) Representative conformer of MNV VPg 11-85, with selected (mainly hydrophobic) side chains shown as sticks and colored by atom type. (E) MNV VPg 11-85 rotated by 110° compared to panel D and with key charged and polar residues involved in electrostatic interactions shown as sticks. Polar interactions are indicated by dashed black lines.