Fig 5.

Effects of pH, NaCl, and temperature on the ¹H-¹⁵N HSQC spectrum of MNV VPg 1-124. (A to C) Spectra recorded for MNV VPg 1-124 at pH 5 (A), pH 6 (B), or pH 7 (C). The spectra were recorded at 303 K with 100 μM protein dissolved in 50 mM malic acid, 2-(N-morpholino)ethanesulfonic acid (MES), and Tris-HCl at a molar ratio of 1:2:2 (MMT), 300 mM NaCl, and 1 mM DTT, which was titrated to the required pH with HCl or NaOH as appropriate. (D to F) ¹H-¹⁵N HSQC spectra recorded at 303 K with 40 μM protein in 50 mM sodium phosphate (pH 6.5) and 1 mM DTT supplemented with 50 mM NaCl (D), 150 mM NaCl (E), or 300 mM NaCl (F). (G to I) ¹H-¹⁵N HSQC spectra recorded with 590 μM protein in 50 mM sodium phosphate (pH 6.5), 300 mM NaCl, and 1 mM DTT at 278 K (G), 293 K (H), or 303 K (I).