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Effects of site-directed mutations on the stability of the core structures of MNV and FCV VPg. The amide region of the 1D 1H NMR spectra of MNV VPg 1-124 (A) and FCV VPg 1-111 (B). In each case, the region of the spectrum shown is the structured amide region of a 1D protein spectrum, which is not overlapped by aromatic signals. The peaks at 10.0 to 10.3 ppm derive from the NHε of Trp side chains.
