Table 3.
Effect of mutations in FCV VPg on virus infectivity
| VPg mutation | Virus recoverya |
|---|---|
| Wild type | +++ |
| H30I | ++ |
| H30A | + |
| F43A | − |
| R47E | − |
| R47G | − |
| F62A | − |
| W65A | − |
| W66A | − |
| R69E | − |
a
Recovery is expressed as the yield 24 h posttransfection relative to that of the wild type (+++) as assayed by >3 independent experiments. Typical yields of wild-type virus were 1 × 104 to 5 × 104 TCID50 units. −, no virus detected; +, up to 100 TCID50 detected; ++, up to 1,000 TCID50 detected; +++, up to WT levels of virus detected.